Human Leukocyte Antigen (HLA)
Human Leukocyte Antigen (HLA) is a protein found on White blood cells. They play a vital role in defending the body against viral or bacterial infections and determine the bodys response to drugs, vaccinations, autoimmune disorders and cancers. HLA types also form the basis for acceptance/ rejection of a transplanted organ/ bone marrow.
BMSTs NABH Accredited, HLA laboratory is one of the foremost testing facilities in India which carries out pre and post- transplant tests for both Bone Marrow Transplant as well as solid organ-Kidney, heart, liver- transplants.
With over 1900 tests in the year 2020 -21, the unique features of the lab are
- One among very few labs in India to use advanced testing techniques for solid organ and bone marrow transplant
- Tests for all cadaver donor transplants in Karnataka
- Initiating latest methods and advocacy among the transplant community has resulted in rational and optimum testing protocols
At the HLA laboratory, the following tests are done:
- CDC Crossmatch Donor & Patient
- DSA Crossmatch
- HLA Typing
- Panel Reactive Antibody Testing
- Single Antigen Test
- HLA Antibody Screening Test
- Flow Cytometric Crossmatch
CDC Cross Match:
Clinical use: The CDC Cross Match is done to detect presence of pre-formed anti-donor HLA antibodies in the serum of potential recipients. The AHG CDC cross match detects these antibodies with greater sensitivity than the routine
Specimen required: Patient serum & donor’s ACD blood sample 20-30 ml
Luminex DSA Cross Match
Clinical use: To detect the presence of antibodies directed against the HLA antigens of the donor & specific HLA IgG antibodies against Class I and/or Class II antigens. It is ideal for pre- and post-transplant testing.
Specimen required: Patient serum (from 5 mL blood sample) & donor ACD blood sample, 20-30 ml
HLA typing AB DR
Clinical use: To identify the Human Leukocyte antigens/ HLA alleles present on the WBCs of the donor / patient for Bone Marrow as well as solid organ transplant
Specimen required: EDTA blood sample 5 ml
PRA / Panel reactive Antibody testing
Clinical use: Class I and II ID panels provide antibody identification and panel-reactive antibody (PRA) information
Specimen required: Patient serum, 5 ml
Single Antigen test
Clinical use: Immunoassays used to qualitatively detect the specificity of HLA, Class I & II IgG antibodies for optimized selection of donor for allosensitized patients especially patients waiting for deceased donor transplant
Specimen required: Patient serum (5 ml)
HLA Antibody Screening Test:
Clinical use: This test detects IgG antibodies to HLA Class I and Class II antigens. The presence or absence of these antibodies has a role in determining the survival of transplant allografts
Specimen required: Patient Serum collected from 4- 5 ml whole blood sample
Flowcytometric Crossmatch:
Clinical use: Flowcytometric crossmatch is a sensitive and accurate test to detect the presence of donor-specific low titre anti-HLA antibodies. his method utilizes a multicolored approach with fluorescently labelled monoclonal antibody to discriminate T-cells from B-cells.
Specimen required: Patient serum & donor’s ACD blood sample 20-30 ml
With our expertise and experience in the field of Transplant Immunology since 1996, we offer clinical correlation & advice for transplant cases
Key Features of BMST HLA Laboratory:
- NABH Accredited
- Luminex based testing – e. automated, higher support for clinical efficacy
- Quick reporting: Results between 24 and 72 hours, as against the usual benchmark of 1 week
- Minimal service charges being a not-for-profit entity
- Review by experts in Transplant Immunology. Each test is reviewed by the Expert Panel on Transplant Immunology to ensure 100% coverage and quality